A package has been created in order to record R functions we usually use in our laboratory for the analysis of amplicon data. Alongside this package, a tutorial was made to show the main utilities of this package, as well as to make public our main workflow (https://nuriamw.github.io/micro4all/). We cover several steps, including:
1. Produce an amplicon sequence variant (ASV) table, making use of dada2 package.
2. Study microbial diversity (α and β diversity).
3. Get taxonomical profiles at phylum and genus level.
4. Differential abundance analysis with ANCOMBC package.
5. Ordination graphics and environmental fitting of soil physicochemical parameters.